Application

Research CategorySignaling

Immunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected CYP7B1 in human cerebral cortex and human pancreatic cancer tissue.

Immunohistochemistry Analysis: A representative lot detected CYP7B1 in in human liver tissue. (Courtesy of Ayham Alnabulsi, Ph.D., Vertebrate Antibodies Ltd. United Kingdom).

Western Blotting Analysis: A 1:2,000 dilution from a representative lot detected CYP7B1 in human liver tissue lysate.

Detect CYP7B1 using this mouse monoclonal Anti-CYP7B1 Antibody, clone M17P3F2, Cat. No. MABS513, used in Immunohistochemistry (Paraffin) and Western Blotting.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

25-hydroxycholesterol 7-alpha-hydroxylase (UniProt: O75881; also known as EC:1.14.13.100, Cytochrome P450 7B1, Oxysterol 7-alpha-hydroxylase) is encoded by the CYP7B1 gene (Gene ID: 9420) in human. CYP7B1 is an enzyme involved in bile acid biosynthesis and shares 40% sequence identity to human cholesterol 7a-hydroxylase (CYP7A1). CYP7B1 localizes to the endoplasmic reticulum membrane and is expressed in brain, testis, ovary, prostate, liver, colon, kidney, and small intestine. It catalyzes the rate-limiting step in the conversion of cholesterol to bile acids. The classic pathway of bile acid synthesis is initiated by cholesterol 7alpha-hydroxylase in the liver and is tightly regulated by bile acid feedback, hormones, and diurnal rhythm. In contrast, the pathway initiated with sterol 27-hydroxylase is located mainly in extrahepatic tissues. An "alternative" pathway of bile acid synthesis begins with 27-hydroxylation of cholesterol by 27-hydroxylase (CYP27), followed by 7alpha-hydroxylation by oxysterol 7alpha-hydroxylase (CYP7B1). Mutations in CYP7B1 have been associated with Spastic paraplegia 5A, an autosomal recessive disorder that is characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs. Disorders in CYP7B1 also cause congenital bile acid synthesis defect 3 (CBAS3) that leads to severe cholestasis, cirrhosis, and liver synthetic failure.

Immunogen

Ovalbumin-conjugated linear peptide corresponding to 9 amino acids from the C-terminal end of human 25-hydroxycholesterol 7-alpha-hydroxylase (CYP7B1).

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Protein G purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Format: Purified

Quality

Evaluated by Immunohistochemistry in human testis tissue.

Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected CYP7B1 in human testis tissue.

Specificity

Clone M17P3F2 detects CYP7B1 in human cerebral cortex, testis, pancreatic cancer, and colon cancer.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

58.26 kDa calculated.